One of the most indispensable techniques in all areas of the life sciences is the use of expression vectors. Cloning a gene between two restriction sites, and the expression of a protein, are almost ...

Until recently, PCR was the only method for direct isolation of desirable chromosomal segments from complex genomes. Although it is widely used for the analysis of genomes, PCR has one major ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...